Biotechnology: Principles and Processes is a Class 12 Biology chapter that carries about 4.1% of the Biology questions in NEET. Practise it with 58 questions covering 2 topics, each with an explanation.
Sets of 15 questions that adapt to your level, with an explanation for every answer.
Across the 13 previous year papers, Biotechnology: Principles and Processes had 56 questions, about 4.3 a year. The most in one paper was 7, in NEET 2021.
| Paper | Questions from this chapter |
|---|---|
| NEET 2026 | 6 |
| NEET 2025 | 5 |
| NEET 2024 | 6 |
| NEET 2023 | 5 |
| NEET 2022 | 6 |
| NEET 2021 | 7 |
| NEET 2020 | 6 |
| NEET 2019 | 2 |
| NEET 2018 | 1 |
| NEET 2017 | 5 |
| NEET 2016 | 3 |
| Re-AIPMT 2015 | 3 |
| AIPMT 2014 | 1 |
8 questions from the practice bank, spread across the chapter's topics. The full set is in the app.
In the annealing step of PCR, the:
Answer: C. primers bind to their complementary sequences on the single-stranded template
Why: This happens at a lower temperature than denaturation.
Restriction endonucleases cut DNA at specific sequences called:
Answer: B. palindromic recognition sites
Why: Most restriction enzymes recognise short palindromic sequences and cleave the DNA at or near that site, generating fragments used in recombinant DNA technology.
The newly synthesised DNA in each PCR cycle is made:
Answer: D. by the polymerase extending the primers using the original and newly made strands as templates
Why: This gives an exponential increase.
The restriction enzyme EcoRI cuts DNA at the palindromic sequence:
Answer: B. GAATTC, giving sticky ends
Why: Sticky ends help the cut fragments join easily with the DNA of the vector.
The ingredients of a PCR mixture include:
Answer: D. template DNA, two primers, the four deoxynucleotides, Taq polymerase and a buffer containing Mg²⁺
Why: Magnesium is a cofactor.
In agarose gel electrophoresis, the smaller DNA fragments:
Answer: A. move faster and farther, as they pass more easily through the pores of the gel
Why: The gel acts as a sieve.
In the extension step of PCR, the temperature is about:
Answer: B. 72 °C, the optimum for Taq polymerase
Why: The enzyme extends the primers using the nucleotides.
In the biolistics or gene gun method, the DNA is introduced into plant cells using:
Answer: B. gold or tungsten particles coated with the DNA
Why: The particles are shot at high velocity.
Biotechnology: Principles and Processes carries about 4.1% of the Biology questions in NEET. Across the 13 previous year papers on NeetVictory it appeared 56 times, about 4.3 questions a year.
Biotechnology: Principles and Processes is a Class 12 Biology chapter in the NCERT syllabus.
NeetVictory has 58 practice questions on Biotechnology: Principles and Processes, each with an explanation, in sets of 15 that adapt to your level. This page shows a free sample.
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