Biotechnology and Its Applications is a Class 12 Biology chapter that carries about 2.1% of the Biology questions in NEET. Practise it with 47 questions covering 4 topics, each with an explanation.
Sample questions on Biotechnology and Its Applications with answers
8 questions from the practice bank, spread across the chapter's topics. The full set is in the app.
Question 1: GM Crops and Bt Cotton
Genetically modified organisms (GMOs) are those whose genes have been:
- A changed only by cross breeding
- B lost through natural selection
- C damaged by radiation always
- D altered by manipulation using recombinant DNA technology
Answer: D. altered by manipulation using recombinant DNA technology
Why: Transgenic plants, animals and microbes are GMOs.
Question 2: GM Crops and Bt Technology
The cry gene that codes for a protein toxic to the cotton bollworm is:
- A cryIIAb
- B cryIAa only
- C cryIAb
- D cryIAc
Answer: D. cryIAc
Why: cryIAb controls the corn borer; cryIAc and cryIIAb control the cotton bollworm.
Question 3: Gene Therapy
Transgenic animals are used to:
- A produce only meat
- B increase the number of chromosomes
- C study how genes are regulated and their effects on the normal functions of the body and its development
- D reduce the size of animals
Answer: C. study how genes are regulated and their effects on the normal functions of the body and its development
Why: They also help to study the diseases and test the safety of vaccines.
Question 4: Gene Therapy and Molecular Diagnostics
The first clinical gene therapy was given in 1990 to a four-year-old girl with:
- A thalassaemia
- B cystic fibrosis
- C ADA deficiency
- D diabetes
Answer: C. ADA deficiency
Why: The lymphocytes were grown outside the body, then returned to the patient.
Question 5: GM Crops and Bt Cotton
The Bt toxin is produced by the bacterium:
- A Agrobacterium tumefaciens
- B Bacillus subtilis
- C Bacillus thuringiensis
- D Escherichia coli
Answer: C. Bacillus thuringiensis
Why: It exists as an inactive protoxin.
Question 6: GM Crops and Bt Technology
The nematode Meloidegyne incognita infects the roots of tobacco plants and causes a reduction in yield. This was prevented by:
- A antibiotics
- B RNA interference (RNAi), using a double-stranded RNA from the nematode genes
- C a herbicide
- D Bt toxin
Answer: B. RNA interference (RNAi), using a double-stranded RNA from the nematode genes
Why: The nematode specific genes were introduced using Agrobacterium vectors.
Question 7: Gene Therapy
The transgenic cow Rosie produced human protein-enriched milk containing:
- A human growth hormone
- B human α-lactalbumin, at 2.4 grams per litre
- C human insulin
- D human α-1-antitrypsin
Answer: B. human α-lactalbumin, at 2.4 grams per litre
Why: The milk was nutritionally a more balanced product for the babies than the natural cow milk.
Question 8: Gene Therapy and Molecular Diagnostics
ELISA and PCR are commonly used molecular techniques for:
- A plant breeding only
- B early diagnosis of diseases like AIDS
- C crop irrigation
- D soil testing only
Answer: B. early diagnosis of diseases like AIDS
Why: ELISA (detecting antigens/antibodies) and PCR (amplifying DNA) are used for early and sensitive diagnosis of diseases such as AIDS.